Interface properties

The hydrophilic and hydrophobic molecules and their interfaces exert an influence on the interface behavior of gelatine. These characteristics determine its properties, such as the stability of its foams and emulsions, its adhesive properties and its solubility.

Amphoteric behavior

Owing to the fact that some amino acids have charged groups attached to their side chains, proteins have different electrical charges. Thus, depending on its amino acid composition, each protein has a different distribution of charge, which is also pH-dependent.

At the isoelectric point (IEP), there are an equal number of positive and negative charges within the molecule. In effect, the molecule is neutral. The isoelectric point of native collagen occurs at approximately pH 9.

Gelatine is produced by the partial hydrolysis of collagen. Type A gelatin, produced using the acid process, has an IEP between 8 and 9. Alkaline-produced gelatine (type B) has an IEP of between 4.8 and 5.4. These differences result from the partial deamination of glutamine and aspargine to glutamic acid and aspergic acid, respectively, during the alkaline pretreatment of the raw materials.

In the application of gelatine, the IEP plays an important role. The nearer the pH of the final product to the IEP of gelatine, the more chance of turbidity or precipitation there is.

Amphiphilic behavior

Gelatine is a polydisperse system comprising different lengths of protein chains that, in turn, consist of long hydrophobic chain segments and short hydrophilic segments. This molecular structure is typical for substances showing surface activity. This is why gelatine is capable of decreasing the surface tension of aqueous systems; gelatine molecules attach themselves to the interface as a film. This ability can be utilized technologically in the formation and stabilization of multi-phase systems such as foams or emulsions.

Foams and emulsions

Depending on the application, a gelatine with particular foaming or whipping qualities may be required. Type A gelatine has better foaming qualities than type B. This at least applies in the pH range 3–6. This can be explained by the different surface charges as a function of pH (amphoteric behavior), which, in turn, leads to a greater or lesser deconvolution of the molecules. Despite these basic differences, both gelatine types are suitable for foaming and stabilization if their molecular design (amphiphilic behavior) is correspondingly adapted. This also applies to the formation and stabilization of emulsions.